Kamis, Mei 28, 2009
• Honey (98 g) + royal jelly (1 g) + propolis (1 g), oral.
• Bee bread + propolis, oral.
• Propolis 30% extract + vitamin C, oral.
Flu, Avian and Swine
• Royal jelly, oral.
• Honey (98 g) + royal jelly (1 g) + propolis (1 g), oral, in star anise (Illicium verum = Anisum stellatum) infusion.
• Honey + garlic extract, nasal drops.
• Honey + garlic extract, oral, with plenty of water.
• Pollen and bee bread, oral.
• Propolis tincture, inhalations.
• Propolis 50% tincture, 20 drops with honey in a water glass before meals.
• Venom sublingual tablet or mixed with honey, oral, supplemented with vitamins C and B-complex.
Also, diet supplementation with all bee products, to drink plenty of water, and to take any other medication prescribed by physician.
• Honey + propolis (1%) + royal jelly (5 - 10%), oral.
• Honey (96 g) + royal jelly (3 g) + propolis (1 g), oral.
• Honey (450 g) + royal jelly (45 g), oral.
source: Apitherapy News
Propolis halted neurofibromatosis tumor growth in a group of cancer patients taking part in a study by scientists at Universitaets Klinikum Eppendorf in Hamburg, Germany. Dysfunction of the NF1 or NF2 gene coding is the major cause of neurofibromatosis. Researchers had previously demonstrated that the human gene PAK1 is essential for the growth of both NF1 and NF2 tumors. Although several attempts have been made to develop anti-PAK1 drugs, none have been successful.
Since bee propolis contains anticancer ingredients caffeic acid phenethyl ester (CAPE) and artepillin C (ARC), both of which block the oncogenic PAK1 signaling pathways, its potential therapeutic effects on NF tumors were explored in vivo. It was demonstrated that a CAPE-rich extract of propolis mixed with water completely suppressed the growth of human NF1 and caused an almost complete regression of human NF2 (Schwannoma), that had been grafted in mice. The scientists stressed that although CAPE is not used clinically due to its poor bioavailability, it can be made soluble with the addition of lipids (fats). (Phytotherapy Resources, February)
In an earlier study, this German team identified CAPE's anti-cancer abilities. CAPE is a natural compound found in some foods, but is highly concentrated in bee propolis. Previously, propolis had been known only to have anti-cancer function through its profound ability to boost the immune system.
Clinical trials to test the compound on humans are ongoing. So far, cancer patients taking part have seen their tumor growth halted…
Propolis is effective against cancer of the larynx
Scientists in Brazil recently investigated the effects of propolis on human laryngeal epidermoid carcinoma. They incubated cells with different concentrations of bee propolis for different time periods. Then they analyzed morphology and number of viable cancerous cells. Their data showed that propolis exhibited the ability to kill cancerous cells in a dose and time dependent manner. (Evidence Based Complementary and Alternative Medicine, October 22, 2007)
Propolis keeps tumors from setting up their own blood supplies
In other recent research, researchers examined the ability of propolis components to stop tumors from developing their own blood supplies. When blood supply to a tumor is cut off, it can no longer receive nutrients to fuel its growth. Acacetin, apigenin, artepillin C, CAPE, chrysin, p-coumaric acid, galangin, kaempherol, pinocembrin, and quercetin were studied for their antioxidant activity as well…
Bee propolis rejuvenates the immune system
The first double-blind study of propolis involved a team of five doctors led by Professor S. Scheller in Poland, who found that propolis had the power to prolong the prime of life by stimulating the immune system to release substances that protect against cellular deterioration. In addition, propolis boosted the destruction of potentially harmful foreign bacteria and stimulated the formation of antibodies to build immunity to many diseases. This strengthening of cellular defense helps build resistance to aging and illness…
By Barbara Minton, 4/29/2009
Senin, April 27, 2009
Propolis has been reported to display a broad spectrum of biological activities such as anticancer, antioxidant, anti-inflammatory, antibiotic and antifungal properties, among others.
There is great interest not only in the determination of the chemical composition of propolis but also in the understanding of the mechanisms related to its therapeutic actions.
In this respect, the aim of the present investigation was to study the influence of both simultaneous (6, 12 and 24 mg/kg b. w.) and subacute (12 mg/kg b. w.) treatment with a crude hydroalcoholic extract of propolis on the frequency of chromosome aberrations induced by the chemotherapeutic agent doxorubicin (DXR) in Wistar rat bone marrow cells…
The results showed that the dose of 12 mg propolis/kg b. w., administered either as a single dose or as subacute treatment, caused a statistically significant decrease in the frequency of chromosome damage induced by DXR compared to the group treated only with DXR. This reduction might be, in part, due to the presence of phenolic compounds in the studied propolis, which are able to capture free radicals produced by chemotherapeutic agents such as DXR.
Clinical and Experimental Dermatology
Background. Cutaneous injury causes a depression in antioxidant status, as reactive oxygen species (ROS) are produced in response to injury.
Aim. To determine the effects of caffeic acid phenethyl ester (CAPE), an antioxidant and anti-inflammatory agent, on wound healing in rats…
Results. Wound tissues showed a significant increase in glutathione and nitric oxide levels, and a significant decrease in malondialdehyde levels and superoxide dismutase levels in the CAPE group compared with the control group. Histopathology of the wound tissues displayed rapid epithelium development in the CAPE group compared with the control group.
Conclusion. This study has demonstrated that CAPE partly accelerates full-thickness wound healing by its antioxidant and ROS-scavenging capabilities.
Bee Propolis is rich in flavonoids, amino acids and minerals. Flavonoids support health by strengthening capillaries and other connective tissue, with an additional function as an anti-inflammatory – a perfect trait for a sore throat.
Integrative Zoology, Volume 3 Issue 4, Pages 311 - 321
Exposure to mercuric chloride (HgCl2; 5 mg kg−1 body weight; i.p.) induced oxidative stress in mice and substantially increased lipid peroxidation (LPO) and oxidized glutathione (GSSG) levels, decreased the level of reduced glutathione (GSH) and various antioxidant enzymes in liver and also increased the activities of liver marker enzymes in serum.
Therapy with propolis extract, a resinous wax-like beehive product (200 mg kg−1 orally, after mercury administration), for 3 days inhibited LPO and the formation of GSSG and increased the level of GSH in the liver. Release of serum transaminases, alkaline phosphatase, lactate dehydrogenase and γ-glutamyl transpeptidase were significantly restored after propolis treatment. The activities of antioxidant enzymes, that is, superoxide dismutase, catalase, glutathione-S-transferase and glucose-6-phosphate dehydrogenase, were also concomitantly restored towards normal levels after propolis administration.
These observations clearly demonstrate that propolis treatment augments antioxidant defense against mercury-induced toxicity and provide evidence that propolis has therapeutic potential as a hepatoprotective agent.
Molecular Nutrition & Food Research, Published Online: 8 Dec 2008
Propolis possesses various physiological activities. In this study, we examined the antiangiogenic and antioxidant activities of various components from propolis: acacetin, apigenin, artepillin C, caffeic acid phenethyl ester, chrysin, p-coumaric acid, galangin, kaempferol, pinocembrin, and quercetin.
The effects of these components were tested on in vitro models of angiogenesis, tube formation and growth of human umbilical vein endothelial cells (HUVECs). Furthermore, these components were evaluated for their antioxidant activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging and ferric reducing/antioxidant power (FRAP) assays.
Two propolis components, caffeic acid phenethyl ester, and quercetin, possessed strong inhibitory effects on tube formation and on endothelial cell proliferation and, coincidentally, showed strong antioxidant activity.
Artepillin C, galangin, and kaempferol also possessed strong antiangiogenic and antioxidant activities to a slightly less degree. In contrast, acacetin, apigenin, and pinocembrin possessed a considerable degree of antiangiogenic activities, although they showed very low antioxidant activities.
From these results, we propose that components from propolis such as artepillin C, caffeic acid phenethyl ester, galangin, kaempferol, and quercetin might represent a new class of dietary-derived antioxidative compounds with antiangiogenic activities. These propolis components may have the potential to be developed into pharmaceutical drugs for the treatment of angiogenesis-dependent human diseases such as tumors.
Arh Hig Rada Toksikol, 2008 Dec 1; 59(4):299-308
Flavonoid components of propolis are biologically active substances with antioxidative, immunostimulative, immunomodulative, and anti-inflamatory properties. The aim of the study was to investigate their cytotoxic effect on different leukaemia cell lines…
The results show different dose- and cell-type-dependent cytotoxicity. Among the flavonoids, quercetin showed the strongest cytotoxic effect in all cell lines. Caffeic acid and chrisyn also expressed a high level of cytotoxicty. Treatment of U937 and HL-60 cell lines with low concentrations of chrisyn or naringenin stimulated cell proliferation.
These results suggest a biphase effect of the tested compounds on monocyte cell lines. Cytotoxicity and growth stimulation mechanisms caused directly by flavonoids should further be investigated on the molecular level.
Authors: Cardile V; Panico A; Gentile B; Borrelli F; Russo A
Department of Physiological Sciences, University of Catania, v.le A. Doria 6, 95125, Catania, Italy
Summary of Research:
Propolis, a natural product derived from plant resins collected by the honeybees, has been used for thousands of years in folk medicine for several purposes.
The extract that contains amino acids, phenolic acids, phenolic acid esters, flavonoids, cinnamic acid, terpenes and caffeic acid, possesses several biological activities such as anti-inflammatory, immunostimulatory, anti-viral and anti-bacterial.
In this study, we assay the effects of propolis extract on the production of key molecules released during chronic inflammatory events as nitric oxide (NO) and glycosaminoglycans (GAGs) in cultures of human cartilaginous tissues and chondrocytes, stimulated with interleukin-1beta (IL-1beta).
We observed that this natural compound and its active principle, caffeic acid phenethyl ester (CAPE), were able to contrast the harmful effects of IL-1beta.
Our data clearly demonstrated the protective action of propolis in cartilage alteration, that appears greater than that elicited by indomethacin, commonly employed in joint diseases.
Journal: Life Sciences
Issue: 2003; 73(8):1027-35
a Protective role of Egyptian propolis against tumor in mice.
Authors: El-khawaga OA; Salem TA; Elshal MF
Chemistry Department, Faculty of Science, Mansoura University, Mansoura City, Egypt. firstname.lastname@example.org
Summary of Research:
BACKGROUND: Propolis has numerous biologic activities including antibiotic, antifungal, antiviral and anti-inflammatory properties. The present work is aimed to study the effect of crude Egyptian propolis on tumor in mice induced by Ehrlich ascitis carcinoma (EAC) cell line.
RESULTS: The administration of propolis (160 mg/kg body weight), by gastric intubation 2 h before the intraperitoneal injection of EAC, effectively inhibited tumor growth and the proliferation of EAC.
The tumor volume was markedly reduced from 7+/-0.9 ml in EAC-infected mice to 1.6+/-0.95 ml in propolis-treated mice. Also, the lipid peroxide level which was 13.3+/-1.24 nmol malodialdehyde (MDA)/mg protein in EAC infected mice was significantly decreased to 3.3+/-2.1 nmol MDA/mg protein.
Reduced glutathione (GSH) and glutathione S-transferase (GST) concentrations were markedly increased in propolis-treated mice. This effect was associated with inhibition of cell cycle progression and induction of apoptosis.
Administration of propolis 2 h before injection of EAC arrested cells in G0/G1 phase and resulted in a decrease in the viability, DNA, total RNA and protein level of tumor cells.
CONCLUSIONS: Crude Egyptian propolis has a strong inhibitory activity against tumors. The anti-tumor mechanism may be mediated by preventing oxidative damage and induction of apoptosis.
Journal: Clinica chimica acta; international journal of clinical chemistry
Issue: 2003; 338(1-2):11-6